“ This targeting vector, which also carried the shv TK sequence, was electroporated into SCR012 ES cells, selected with G418 and GanC to reduce random integration and then screened for homologous recombinants by PCR and Southern blot analysis stromectol in canada In both DAT Cre and DAT CreERT2 mice, this seemingly ectopic reporter gene expression was made using the Ai14 reporter line in which the reporter, the tdTOM fluorescent protein, labels both cell bodies and projections ”