“ C eMLPA Supplemental Figure 1 on DNA from kidney samples; mice that carry two Pkd1 lox alleles iKsp Pkd1 loxlox; high dose, n 7; low dose, n 12 contain two times the amount of Pkd1 del DNA compared with mice that carry a single Pkd1 lox allele iKsp Pkd1 lox, wt; high dose, n 9; low dose, n 6, indicating that, when Cre is activated in cells with two floxed Pkd1 alleles, both alleles will be recombined to create Pkd1 del alleles lasix side effects on kidneys Our cytotoxicity panel provides the next tier of information on subcellular mechanisms and potential adverse effects compared to a single assay ”